Dr. Sen Hou
Name : Sen Hou
Gender : Male
Date of Birth : Dec 7, 1981.
Marital Status : Married
Occupation : assistant professor
E-mail : firstname.lastname@example.org
Contact Address : Department
To obtain a challenging position in multiple fields including research about micro-fluidic system, research about microcontact printing technique, advanced materials for surface modification, DNA condensation research, gene delivery vector research, research in biosensors, application of fluorescence correlation spectroscopy in bioassay and biointerface.
Major in life science 2000-2004
Major in biochemistry and molecular biology 2004-2009
Application of fluorescence correlation spectroscopy in bioassay in crowded environment 2009-present
PUBLICATIONS IN ENGLISH
 Sen Hou, Kun Yang, Ming Qin, Xi-Zeng Feng, Li Guan, Yanlian Yang, ChenWang. Patterning of cells on functionalized poly(dimethylsiloxane) surface prepared by hydrophobin and collagen modification. Biosensors and Bioelectronics 2008, 24, 918–922
 Sen Hou, Kun Yang, Xi-Zeng Feng. Explanation for the electrophoresis behaviour of DNA condensation induced by pseuduopolyrotaxane of different lengths. Electrophoresis 2008, 29, 4391-4398.
 Sen Hou, Kun Yang, Ze Liu, Xi-Zeng Feng. A method to increase the bioactivity of plasmid DNA by heat treatment. J. Biochem. Biophys. Methods 2008, 70, 1066–1072.
 Sen Hou, Kun Yang, Yang Yao, Ze Liu, Xizeng Feng, Rui Wang, Yanlian Yang, Chen Wang. DNA condensation induced by a cationic polymer studied by atomic force microscopy and electrophoresis assay. Colloids and Surfaces B: Biointerfaces 2008, 62, 151-156.
 Yu Liu, Yong Chen, Zhong-Yu Duan, Xi-Zeng Feng, Sen Hou, Chen Wang, Rui Wang. A Luminescent β-Cyclodextrin-Based Ru(phen)3 Complex as DNA Compactor, Enzyme Inhibitor, and Translocation Tracer. ACS Nano 2007, 1, 313-318.
 Ming Qin, Sen Hou, LiKai Wang, XiZeng Feng, Rui Wang, YanLian Yang, Chen Wang b, Lei Yu, Bin Shao, MingQiang Qiao. Two methods for glass surface modification and their application in protein immobilization. Colloids and Surfaces B: Biointerfaces 2007, 60, 243–249.
 Chen-Feng Ke, Sen Hou, Heng-Yi Zhang, Yu Liu, Kun Yang, Xi-Zeng Feng. Controllable DNA condensation through cucurbituril in 2D pseudopolyrotaxanes. Chem. Commun. 2007, 3374–3376.
 S. Hou, X.Z. Feng, L. K. Wang, Q. L.
Chan. Microcontact printing of multiproteins on the GA modified glass
substrate and its applications in the research of immunoassays.
 Likai Wang, Xizeng Feng, Sen Hou, Qinlin Chan, Ming Qin. Microcontact printing of multiproteins on the modified mica substrate and study of immunoassays. Surf. Interface Anal. 2006, 38, 44-50.
 Liu Tao, Chen Long, Hou Sen, Xue Yonglai, Feng Xizeng. Non-viral gene delivery carrier of probe type host molecule ——Interactions between DNA and β-cyclodextrin derivative complexes (I). Chinese Science Bulletin, 2006, 51, 530-535.
 Feng Xizeng, Hou Sen, Wang Likai, Chan Qilin, Han Peidong. Microfluidic networks techniques in biosciences, Chemical Journal on Internet, 2004, 6, 62-62.
 Feng Xi-zeng, Hou Sun, Chan Qi-lin, Wang Li-kai, Qin Ming, Han Pei-dong. Microcontact printing techniques in bioscience. Chem. Res. Chinese U. 2004, 20, 826-832.
 Sen Hou, Xinxin Li, Xiaoyu Li, Xi-Zeng Feng, Rui Wang, Chen Wang, Lei Yu, Ming-Qiang Qiao. Surface modification using a novel type I hydrophobin HGFI Anal Bioanal Chem 2009, 394, 783–789.
 Yong Chen, Lu Yu, Xi-Zeng Feng, Sen Hou, Yu Liu. Construction, DNA wrapping and cleavage of a carbon nanotube–polypseudorotaxane conjugate. Chem. Commun. 2009 4106-4108.
 Sen Hou, Xin-Xin Li, Xiao-Yu Li, Xi-Zeng Feng, Rui Wang, Chen Wang, Lei Yu, Ming-Qiang Qiao. Patterning of 293T fibroblasts on a mica surface. Anal Bioanal Chem 2009, 394, 2111-2117.
 Sen Hou, Xinin Li, Xi-Zheng Feng. Method to Improve DNA Condensation Efficiency by Alkali Treatment. Nucleosides, Nucleotides & Nucleic Acids 2009, 28, 1532-2335
 Xinxin Li,
Sen Hou, Xizeng Feng, Yong Yu,
Jingjian Ma, Lanying Li. Patterning of neural stem cells on
poly(lactic-co-glycolic acid) film modified by hydrophobin. Colloids and Surfaces B
 Porous film fabricated by a thermoresponsive polymerpoly (N-isopropylacrylamide-co-butylmethacrylate) with enhanced hydrophobicity. Sen Hou, Yongzheng Ma, Xinxin Li, Xizeng Fenga, Yudong Zhang,Xiaokun Dong, Yongchun Fang. Colloids and surface A 2009, 246, 164-169
Ma, Sen Hou, Bin Ji, Yang
 Lei Liu, Xinxin Li, Sen Hou, Yonglai Xue, Yang Yao, Yongzheng Ma, Xizeng Feng, Song He, Yan Lu, Yongmei Wang, Xianshun Zeng. Nanoparticles from Block Copolymer Encapsulating Re(phen) complexes as Bifunctional Agents for Cell Imaging and Gene Transfection. Chem Comm. 2009, in press.
 Sen Hou, Xinxin Li, Xiaoyu Li, Xizeng Feng. Coating of hydrophobins on three-dimensional electrospun poly(lactic-co-glycolic acid) scaffolds for cell adhesion. Biofibrication 2009, in press.
 Lei Liu,
Yan-Lian Yang, Chen Wang, Yang
PUBLICATIONS IN CHINESE
 Li Xinxin, Hou Sen, Feng Xi-zeng. Progress of inorganic nanoparticlesas gene delivery vectors. Chinese Bulletin of Life Sciences 2008, 20, 402-407.
 Li Xiaoyu, Hou Sen, Feng Xi-zeng. Application of microfluidic technology in cytology. Chinese Bulletin of Life Sciences 2008, 20, 319-401.
 Yao Yang, Hou Sen, Feng Xi-zeng. Design and optimization of polymers for gene delivery. Chinese Journal of Clinical Rehabilitation 2006, 10, 125-129.
 Yang Kun, Hou Sen, Feng Xi-zeng. The flexibility of sharply bent DNA. ACTA Biophysica Sinica 2006, 22, 251-258.
 Liu Tao, Chen Long, Hou Sen, Xue Yonglai, Feng Xizeng. Applications of a host molecule non-viral gene delivery carrier. Chinese Science Bulletin, 2006, 51, 399-403.
 Hou Sen, Han Peidong, Feng Xizeng. Microcontact Printing Using in Biomicroanalytics. Chemistry 2005, 68, w065.
 Xue Yonglai, Feng Xizeng, Hou Sen. Application of single-molecule manipulation techniques to the study of nucleic acids. Chemistry 2005, 8, 601-607.
 Yu Yong, Li
Yanying, Ma jingjian, Hou Sen, Li
Xinxin, Feng Xizeng. Influence of hydrophobin HFBI and collagen modification
on the adhesion and growth of neuron stem cells.
2000 Awarded the Second–Class Scholarship of
2001 Awarded the Third–Class Scholarship of
2004 Awarded the Outstanding dissertation of
2005 Awarded the Third–Class Scholarship of
2006 Awarded the Third–Class Scholarship of
2007 Awarded the
Second–Class Scholarship of
2008 Awarded the First–Class Scholarship of
2008 Awarded Merit Student of
I have been engaged in studying the field of gene delivery vector since I came into being a PhD candidate of Prof. Feng. In the beginning, I mainly investigated the interactions between DNA and DNA condensate, which was defined as the materials that can compact DNA into highly ordered structure.
After about half a year, I found that the highly ordered DNA structure had a comprehensive influence on their biological functions and I began to investigate the relation between DNA condensation and their functions.
During this period, I also did researches in the gene delivery vector field in cooperation with Prof. Liu. I was in charge of all of the biological experiments. Up to now I have mastered nearly all of the biological experiments in this field ranging from DNA purification to gene transfection in mammal cells.
Surface modification on the biointerfaces is another my research field. In the beginning, I was engaged in the surface modification using microcontact printing technique for immunoassay. The technique mainly based on chemical or biological materials for surface modification.
Then I found the surface property had influence on the cell adhesion behavior and I started to do some research on mammal cell patterning on the surfaces. The cells include NIH 3T3, 293T fibroblast cells and even neural stem cells.
I have also performed some researches about microfluidic system. I want to investigate the behavior of life in a narrow channel. I have studied the cell’s behavior with different flow rate, the DNA condensation process in a T shape channel and the influence of microfluidic system on the gene transfection efficiency. Combinative study of biointerface and gene vector is my ultimate goal.
Enrolled in a TEAM project (2009-2013) From nano to macroscale: motion of proteins, protein charge ladders and nanoparticles in complex liquids and diffusion limited reactions in crowded environment
Have conducted experiments:
Microcontact printing technique, Microfluidic system, DNA purification, mammalian cell culture, bacteria culture, gene transfection in mammalian cells, EB displacement assay, cell toxicity assay, sample preparation for AFM and SEM observation, electrophoresis assay, fluorescence correlation spectroscopy and other ordinary biological experiments.
Can analyze the reports of equipments:
X-ray Photoelectron Spectroscopy (XPS), circular dichroism spectrometer (CD), Atomic Force Microscope (AFM), Scanning Tunneling Microscopy (STM), Scanning Electron Microscope (SEM), fluorescence correlation spectroscopy and Transmission Electron Microscope (TEM).
the 2nd Korean-Chinese Students Workshop
Oral presentation: “Bio-surface modification with hydrophobins for protein immobilization”
Sons Meeting, 6-8 July 2009,