Department III,
Dr. Sen
Hou
Personal Data
Name : Sen Hou
Gender : Male
Nationality :
Date of Birth : Dec
7, 1981.
Marital Status : Married
Occupation : assistant
professor
E-mail : hs0010910@gmail.com
Contact Address : Department
III,
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OBJECTIVE |
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To obtain a challenging
position in multiple fields including research about micro-fluidic system,
research about microcontact printing technique, advanced materials for
surface modification, DNA condensation research, gene delivery vector
research, research in biosensors, application of fluorescence correlation
spectroscopy in bioassay and biointerface. |
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EDUCATION |
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B.S. Major in life
science 2000-2004 Ph.D. Major in
biochemistry and molecular biology 2004-2009 Assistant professor Application of fluorescence correlation spectroscopy in
bioassay in crowded environment 2009-present Department III, |
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PUBLICATIONS IN ENGLISH |
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[1] Sen Hou, Kun Yang,
Ming Qin, Xi-Zeng Feng, Li Guan, Yanlian Yang, ChenWang. Patterning of cells
on functionalized poly(dimethylsiloxane) surface prepared by hydrophobin and
collagen modification. Biosensors and
Bioelectronics 2008, 24,
918–922 [2] Sen Hou,
Kun Yang, Xi-Zeng Feng. Explanation for the electrophoresis behaviour of
DNA condensation induced by pseuduopolyrotaxane of different lengths. Electrophoresis 2008, 29,
4391-4398. [3] Sen Hou, Kun Yang, Ze Liu, Xi-Zeng
Feng. A method to increase the bioactivity of plasmid DNA by heat treatment. J. Biochem. Biophys. Methods 2008, 70, 1066–1072. [4] Sen Hou, Kun Yang,
Yang Yao, Ze Liu, Xizeng Feng, Rui Wang, Yanlian Yang, Chen Wang. DNA
condensation induced by a cationic polymer studied by atomic force microscopy
and electrophoresis assay. Colloids and
Surfaces B: Biointerfaces 2008,
62, 151-156. [5] Yu Liu,
Yong Chen, Zhong-Yu Duan, Xi-Zeng Feng, Sen
Hou, Chen Wang, Rui Wang. A Luminescent β-Cyclodextrin-Based Ru(phen)3
Complex as DNA Compactor, Enzyme Inhibitor, and Translocation Tracer. ACS Nano 2007, 1, 313-318. [6] Ming Qin, Sen Hou, LiKai Wang, XiZeng Feng, Rui
Wang, YanLian Yang, Chen Wang b, Lei Yu, Bin Shao, MingQiang Qiao. Two
methods for glass surface modification and their application in protein
immobilization. Colloids and Surfaces
B: Biointerfaces 2007, 60,
243–249. [7] Chen-Feng
Ke, Sen Hou, Heng-Yi Zhang, Yu
Liu, Kun Yang, Xi-Zeng Feng. Controllable DNA condensation through
cucurbit[6]uril in 2D pseudopolyrotaxanes. Chem. Commun. 2007,
3374–3376. [8] S. Hou, X.Z. Feng, L. K. Wang, Q. L.
Chan. Microcontact printing of multiproteins on the GA modified glass
substrate and its applications in the research of immunoassays. [9] Likai Wang,
Xizeng Feng, Sen Hou, Qinlin Chan,
Ming Qin. Microcontact printing of multiproteins on the modified mica
substrate and study of immunoassays. Surf.
Interface Anal. 2006, 38,
44-50. [10] Liu Tao, Chen
Long, Hou Sen, Xue Yonglai, Feng
Xizeng. Non-viral gene delivery carrier of probe type host molecule
——Interactions between DNA and β-cyclodextrin derivative complexes (I). Chinese Science Bulletin, 2006, 51, 530-535. [11] Feng
Xizeng, Hou Sen, Wang Likai, Chan
Qilin, Han Peidong. Microfluidic networks techniques in biosciences, Chemical Journal on Internet, 2004, 6, 62-62. [12] Feng
Xi-zeng, Hou Sun, Chan Qi-lin,
Wang Li-kai, Qin Ming, Han Pei-dong. Microcontact printing techniques in
bioscience. Chem. Res. Chinese U. 2004, 20, 826-832. [13] Sen Hou, Xinxin Li, Xiaoyu Li,
Xi-Zeng Feng, Rui Wang, Chen Wang, Lei Yu, Ming-Qiang Qiao. Surface
modification using a novel type I hydrophobin HGFI Anal Bioanal Chem 2009, 394, 783–789. [14] Yong Chen, Lu Yu, Xi-Zeng Feng,
Sen Hou, Yu Liu. Construction, DNA wrapping and cleavage of a carbon
nanotube–polypseudorotaxane conjugate. Chem.
Commun. 2009 4106-4108. [15] Sen Hou, Xin-Xin Li, Xiao-Yu Li,
Xi-Zeng Feng, Rui Wang, Chen Wang, Lei Yu, Ming-Qiang Qiao. Patterning of
293T fibroblasts on a mica surface. Anal
Bioanal Chem 2009,
394, 2111-2117. [16] Sen Hou, Xinin Li, Xi-Zheng Feng.
Method to Improve DNA Condensation Efficiency by Alkali Treatment. Nucleosides, Nucleotides & Nucleic
Acids 2009, 28, 1532-2335 [17] Xinxin Li,
Sen Hou, Xizeng Feng, Yong Yu,
Jingjian Ma, Lanying Li. Patterning of neural stem cells on
poly(lactic-co-glycolic acid) film modified by hydrophobin. Colloids and Surfaces B [18] Porous
film fabricated by a thermoresponsive polymerpoly (N-isopropylacrylamide-co-butylmethacrylate)
with enhanced hydrophobicity. Sen Hou,
Yongzheng Ma, Xinxin Li, Xizeng Fenga, Yudong Zhang,Xiaokun Dong, Yongchun
Fang. Colloids and surface A 2009, 246, 164-169 [19] Yongzheng
Ma, Sen Hou, Bin Ji, Yang [20] Lei Liu,
Xinxin Li, Sen Hou, Yonglai Xue,
Yang Yao, Yongzheng Ma, Xizeng Feng, Song He, Yan Lu, Yongmei Wang, Xianshun
Zeng. Nanoparticles from Block Copolymer Encapsulating Re(phen) complexes as
Bifunctional Agents for Cell Imaging and Gene Transfection. Chem Comm. 2009, in press. [21] Sen Hou, Xinxin Li, Xiaoyu Li, Xizeng
Feng. Coating of hydrophobins on three-dimensional electrospun
poly(lactic-co-glycolic acid) scaffolds for cell adhesion. Biofibrication 2009, in press. [22] Lei Liu,
Yan-Lian Yang, Chen Wang, Yang PUBLICATIONS IN CHINESE |
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[1] Li Xinxin, Hou Sen, Feng Xi-zeng. Progress of
inorganic nanoparticlesas gene delivery vectors. Chinese Bulletin of Life Sciences 2008, 20, 402-407. [2] Li Xiaoyu, Hou Sen, Feng Xi-zeng. Application of microfluidic technology in
cytology. Chinese Bulletin of Life
Sciences 2008, 20, 319-401. [3] Yao Yang, Hou Sen, Feng
Xi-zeng. Design and optimization of polymers for gene delivery. Chinese Journal of Clinical Rehabilitation
2006, 10, 125-129. [4] Yang Kun, Hou Sen, Feng Xi-zeng. The
flexibility of sharply bent DNA. ACTA
Biophysica Sinica 2006, 22,
251-258. [5] Liu Tao, Chen
Long, Hou Sen, Xue Yonglai, Feng
Xizeng. Applications of a host molecule non-viral gene delivery carrier. Chinese Science Bulletin, 2006, 51, 399-403. [6] Hou Sen, Han Peidong, Feng
Xizeng. Microcontact Printing Using in Biomicroanalytics. Chemistry 2005, 68, w065. [7] Xue
Yonglai, Feng Xizeng, Hou Sen.
Application of single-molecule manipulation techniques to the study of nucleic
acids. Chemistry 2005, 8, 601-607. [8] Yu Yong, Li
Yanying, Ma jingjian, Hou Sen, Li
Xinxin, Feng Xizeng. Influence of hydrophobin HFBI and collagen modification
on the adhesion and growth of neuron stem cells. |
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AWARDS |
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RESEARCH
EXPERIENCE |
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I have been engaged in studying the field of gene delivery vector
since I came into being a PhD candidate of Prof. Feng. In the beginning, I
mainly investigated the interactions between DNA and DNA condensate, which
was defined as the materials that can compact DNA into highly ordered
structure. After about half a year, I found that the highly ordered DNA structure
had a comprehensive influence on their biological functions and I began to
investigate the relation between DNA condensation and their functions. During this period, I also did researches in the gene delivery
vector field in cooperation with Prof. Liu. I was in charge of all of the
biological experiments. Up to now I have mastered nearly all of the
biological experiments in this field ranging from DNA purification to gene
transfection in mammal cells.
Surface modification on
the biointerfaces is another my research field. In the beginning, I was engaged
in the surface modification using microcontact printing technique for
immunoassay. The technique mainly based on chemical or biological materials
for surface modification. Then I found the surface
property had influence on the cell adhesion behavior and I started to do some
research on mammal cell patterning on the surfaces. The cells include NIH
3T3, 293T fibroblast cells and even neural stem cells.
I have also performed some
researches about microfluidic system. I want to investigate the behavior of
life in a narrow channel. I have studied the cell’s behavior with different
flow rate, the DNA condensation process in a T shape channel and the
influence of microfluidic system on the gene transfection efficiency.
Combinative study of biointerface and gene vector is my ultimate goal.
Enrolled in a TEAM project
(2009-2013) From nano to macroscale: motion of proteins, protein charge
ladders and nanoparticles in
complex liquids and diffusion limited reactions in crowded environment |
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SKILLS |
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Microcontact printing
technique, Microfluidic system, DNA purification, mammalian cell culture,
bacteria culture, gene transfection in mammalian cells, EB displacement
assay, cell toxicity assay, sample preparation for AFM and SEM observation,
electrophoresis assay, fluorescence correlation spectroscopy and other
ordinary biological experiments.
X-ray Photoelectron
Spectroscopy (XPS), circular dichroism spectrometer (CD), Atomic Force
Microscope (AFM), Scanning Tunneling Microscopy (STM), Scanning Electron
Microscope (SEM), fluorescence correlation spectroscopy and Transmission
Electron Microscope (TEM). |
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Academic Activities |
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Oral
presentation: “Bio-surface modification with hydrophobins for protein
immobilization”
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