The confocal imaging lab features a comprehensive system, based on a Nikon A1 inverted microscope. It offers fast and detailed imaging of fluorescent samples, as well as time-correlated single-photon counting capability.
A total of 7 laser lines covers most of the visible light spectrum, with detection in up to 4 channels in parallel. Piezoelectric table allows for extremely precise scanning along the z axis, while advanced software enables full 3D reconstruction of the sample projection from confocal slices. A pulsed laser combined with single photon counting system can be used for fluorescence lifetime imaging as well as fluorescence correlation spectroscopy measurements. It is also possible to perform fluorescence recovery after photobleaching and Forster resonance studies.
Apart from regular photomultiplies as well as single photon avalanche photodiodes, the system comprises a spectral detector and a transmitted light detector. With a range of top-tier immersion and dry objectives as well as active vibration damping, the confocal imaging lab offers the ultimate equipment for most kinds of fluorescence-based experimental studies.
The laboratory operates within Ultrafast Microfluidic Devices Laboratory.