Created at the beginning of 2014 this laboratory is designated place for cultures of eukaryotic cells. It is equipped with all necessary instruments to grow and experiment on every commonly used human and animal cell line. Microinjection setup installed allows precise and efficient introduction of desired probes into living cells, preparing them for following confocal measurements.
Laboratory is located in a separated room with air purification system to provide sterile conditions. Additionally water purification system is present. It is equipped with two CO2 incubators (Nuaire NU-5810 E), laminar flow chamber (2nd class, Thermo Scientific MSC Advantage 1.5) and small equipment for sterile liquid handling. Two microscopes are in use for biological purposes: upright microscope (Nikon Eclipse E200) and inverted fluorescence microscope (Nikon Eclipse Ti-S) coupled with CCD camera and image acquisition system. Any additional small equipment required for basic cell procedures is accessible. This includes centrifuges, water bath, heating and cooling blocks.
We have set of Narishige NT-88-V3 micromanipulators for handling and puncturing of individual cells. Microinjections are performed using Eppendorf FemtoJet pump. This setup enables injection of femtoliter amounts of probes into living adherent mammalian cells. Glass microneedles are pulled with P-1000 Micropipette Puller (Sutter Instrument).
We have cell line bank stored in liquid nitrogen. Currently our laboratory has in its collection: two HeLa (human cervical cancer) lines, unmodified one and one with stable eGFP expression, HepG2 (liver hepatocellular carcinoma) line and human fibroblasts.